Journal: Journal of food and drug analysis

Article Title: Suppression of ERK1/2 and hypoxia pathways by four Phyllanthus species inhibits metastasis of human breast cancer cells.

doi: 10.1016/j.jfda.2016.03.010

Figure Lengend Snippet: Figure 2 e Protein-expression levels in (A) untreated cells and (B) representative images of cells treated with aqueous Phyllanthus watsonii. (C) Western blot showing VEGF expression in untreated cells and cells treated with extracts from the four Phyllanthus species. (d) Percentage of individual protein expression. (E, F) MMP expression in cells after treatment with aqueous and methanolic extracts from four Phyllanthus species, respectively. ELISA-based detection of (G) iNOS and (H) VEGF expression in untreated cells treated with extracts from the four Phyllanthus species. Error bars indicate the standard error of the mean of three independent experiments. p < 0.05 for each Phyllanthus treatment as compared with the untreated group. APN ¼ aqueous P. niruri; APU ¼ aqueous P. urinaria; APW ¼ aqueous P. watsonii; APA ¼ aqueous P. amarus; C ¼ untreated control; Cis ¼ cisplatin; Dox ¼ doxorubicin; ELISA ¼ enzyme-linked immunosorbent assay; H ¼ 500 mg/mL; I ¼ IC50 dosage; iNOS ¼ inducible nitric oxide synthase; L ¼ 50 mg/mL; M ¼ DNA marker; MMP ¼ matrix metalloproteinase; MPA ¼ methanolic P. amarus; MPN ¼ methanolic P. niruri; MPU ¼ methanolic P. urinaria; MPW ¼ methanolic P. watsonii; VEGF ¼ vascular endothelial growth factor.

Article Snippet: Total iNOS in the four Phyllanthus species-treated cells was measured using a human total-iNOS immunoassay enzymelinked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN, USA) according to manufacturer instructions.

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Control, Marker

Journal: Neuron

Article Title: Edinger-Westphal peptidergic neurons enable maternal preparatory nesting

doi: 10.1016/j.neuron.2022.01.012

Figure Lengend Snippet:

Article Snippet: For circulating Progesterone titration, a drop of blood was sampled from the mouse cheek, and processed through a Mouse Progesterone ELISA kit according to the manufacturer’s instructions (Novus Biological NBP2-60125-1).

Techniques: Virus, Recombinant, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: Cancer immunology research

Article Title: Antitumor effects of CAR T cells redirected to the EDB splice variant of fibronectin

doi: 10.1158/2326-6066.CIR-20-0280

Figure Lengend Snippet: (A) Scheme of retroviral vectors encoding the EDB-CAR, a 2A sequence, and truncated CD19 (tCD19); SH: short hinge; TM: transmembrane domain. (B-C) EDB-CAR expression was determined on T cells 7 days post-transduction by flow cytometric analysis. (B) Representative histogram (Black-filled line: NT T cells; Red-filled line: EDB-CAR transduced T cells) and (C) summary plot (n=7; Student’s t-test; ****p<0.0001). (D) NT and EDB-CAR T cells were incubated for 24 hours with increasing concentrations of rhFN-EDB-coated wells. IFNγ production in supernatants was determined by ELISA (n=4; two-way ANOVA; ***p<0.001; ****p<0.0001). (E) EDB expression of LM7, A673, A549, and U87 tumor cells, and two primary fibroblast cell lines (Fib 1, Fib 2) determined by RT-qPCR; dotted line represents the ΔCt score above which samples are considered positive. (F) NT and EDB-CAR T cells were incubated for 48 hours with EDB-positive tumor cells. IFNγ and IL2 production in supernatants was determined by ELISA (two-way ANOVA; ****p<0.0001). (G) Cytolytic activity of NT or EDB-CAR T cells at a E:T ratio of 4:1 against EDB-positive tumor cells (MTS assay; n=3; two-way ANOVA; *p<0.05; **p<0.01; ***p<0.001). (H) Cytolytic activity of NT or EDB-CAR T cells against primary fibroblasts (data of Fib 1 and Fib 2 was combined) at the indicated E:T ratios, with A549 serving as controls (n=3; two-way ANOVA; ****p<0.0001). (I) NT, EDB-CAR, or mutEDB-CAR T cells were incubated for 48 hours with U87 or U87FN−/− cells. IFNγ production in supernatants was determined by ELISA (n=3; two-way ANOVA; ****p<0.0001). (J) Cytolytic activity of NT, EDB-CAR, or mutEDB-CAR T cells against U87 or U87FN−/− cells (n=3; two-way ANOVA; ****p<0.0001). Mean+SEM is shown in panels.

Article Snippet: Cytokines were measured using human IFNγ and IL2 ELISA kits (R&D Systems).

Techniques: Retroviral, Sequencing, Expressing, Transduction, Incubation, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Activity Assay, MTS Assay

Journal: Journal of Clinical Immunology

Article Title: Mice with Asthma Are More Resistant to Influenza Virus Infection and NK Cells Activated by the Induction of Asthma Have Potentially Protective Effects

doi: 10.1007/s10875-011-9619-2

Figure Lengend Snippet: Asthmatic model mice exhibit increased OVA-specific IgE in their serum and histopathological changes characterized by allergic asthma. C57BL/6 mice were intraperitoneally sensitized with OVA or PBS as a control every other day for 2 weeks, then allowed to rest for 3 weeks. The OVA-sensitized mice or control mice were then intranasally challenged with OVA or PBS on days −3, −2 and −1, respectively. Bloods, trachea tissues, and lungs were collected from the mice on day 0. a The OVA-specific IgE in the serum was measured by an ELISA assay. b The trachea tissues ( upper panels ) and lungs ( bottom panels ) were stained with HE. Six mice were used in each group and similar observations were obtained from each mouse

Article Snippet: The cytokine concentrations in the BALF supernatants were determined using a mouse Interferon Alpha ELISA Kit (PBL Biomedical Laboratories, NJ, USA) and a mouse IL-12p40 ELISA development kit, mouse IFN-γ ELISA development kit, mouse IL-6 ELISA development kit, mouse TNF-α ELISA development kit, mouse IL-4 ELISA development kit, mouse IL-13 ELISA development kit, or a mouse IL-22 ELISA development kit (R&D Systems, MN, USA) according to the manufacturer’s instructions, as described previously [ ].

Techniques: Control, Enzyme-linked Immunosorbent Assay, Staining

Journal: Journal of Clinical Immunology

Article Title: Mice with Asthma Are More Resistant to Influenza Virus Infection and NK Cells Activated by the Induction of Asthma Have Potentially Protective Effects

doi: 10.1007/s10875-011-9619-2

Figure Lengend Snippet: Differences were observed in the cytokine production between asthmatic mice and control mice during influenza virus infection. C57BL/6 mice were sensitized and challenged with OVA or PBS. Subsequently, the mice were infected with 100 pfu of the influenza virus. BALF samples from the mice were collected on day 0 before viral infection and on days 2, 4, and 6 after influenza virus infection, and the cytokine concentrations in the BALF supernatants were measured by ELISA assays. Six mice were used in each group. These results are representative of two independent experiments. * p < 0.05 compared to the cytokine production of control mice. These results are representative of two independent experiments

Article Snippet: The cytokine concentrations in the BALF supernatants were determined using a mouse Interferon Alpha ELISA Kit (PBL Biomedical Laboratories, NJ, USA) and a mouse IL-12p40 ELISA development kit, mouse IFN-γ ELISA development kit, mouse IL-6 ELISA development kit, mouse TNF-α ELISA development kit, mouse IL-4 ELISA development kit, mouse IL-13 ELISA development kit, or a mouse IL-22 ELISA development kit (R&D Systems, MN, USA) according to the manufacturer’s instructions, as described previously [ ].

Techniques: Control, Virus, Infection, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Microglial synaptic pruning in the nucleus accumbens during adolescence sex-specifically influences splenic immune outcomes

doi: 10.1101/2023.05.03.539317

Figure Lengend Snippet: In remaining tissue (unpooled) that underwent proteomic analysis ( , ) we conducted ELISAs to better understand splenic changes in response to manipulating NAc development via measurement of markers of neurochemical systems broadly important for splenic function: acetylcholine (ACh), noradrenaline (NA), and tyrosine hydroxylase (TH). Inhibiting pruning in the NAc incresaed (A) ACh and (B) NA levels in the spleen in females, but not males. NIF treatment did not statistically significantly change TH levels in either sex. In each histogram, horizontal lines are average and vertical lines are standard error of the mean. n =7-8/sex/condition. * p <0.05.

Article Snippet: ACh and TH ELISAs were purchased from Novus Biologicals (#NBP2-66389 and #NBP3-06922, respectively).

Techniques: